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Antibody and Bioresource Core Facility

 

The Antibody and Bioresource Core Facility is a

Bi-institutional shared resource supporting researchers at

Memorial Sloan Kettering Cancer Center

and The Rockefeller University

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REMEMBER:

When publishing work done with or by the core, please

acknowledge that this shared resource is supported in part by the

NCI Cancer Center Support Grant (2 P30 CA008748-53)

and include the core's Research Resource Identifier (RRID: SCR_017691)

 

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Services and Support

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Custom Monoclonal Antibody Development
 

Researchers who cannot find an antibody that meets their scientific needs can partner off with the core to develop a strategy to generate custom monoclonal antibodies (MAbs). The facility is well equipped to:

  • Provide expertise built on 20 years of experience
  • Oversee new MAbs projects, as well as the validation of the MAb developed
  • Immunize mice, rats and hamsters
  • Create 10's of thousands of hybridomas by electrofusion
  • Isolate the 1 - 15 % of IgG secreting hybridomas from the electrofusion product to minimize screening efforts and maintain them during screening
  • Perform ELISAs
  • Cryopreserve and store of hybridomas secreting antigen specific antibodies
  • Subclone hybridoma cultures

The core does not develop polyclonal antibodies at this time but is available to provide advice free of charge.

With respect to general validation of antibodies, the core is here to lead researchers through the process and direct them to application experts in other core facilities as needed.

 

To speak with someone in the core about starting a custom MAb project, contact Frances Weis-Garcia by email or call 646-888-2354.

 

Monoclonal Antibodies

Monoclonal antibodies are available in various forms:

  • Conditioned Media:
    • Media from a hybridoma cultured at low density in a T flask
    • Typical MAb Concentration ~ 10 ug / ml
  • Bioreactor Supernatants:
    • Media from a hybridoma cultured in a high density bioreactor
    • Typical MAb Concentration > 1 mg / ml
    • Smallest Aliquot Size = 5 mg (when in stock)
    • Contains:
      • Hybridoma-SFM (Invitrogen)
      • 0.5% fetal bovine serum (prescreened for ultra low bovine Ig levels)
      • Cell debris (from the hybridoma)
    • Does NOT contain sodium azide
    • Bioreactor supernatants can be used in most assays (i.e., western blots, immunoprecipitations, FACS analysis, in vivo depletion, etc.)
  • Purified MAbs:
    • MAb concentration > 1 mg / ml
    • Purity > 95%
    • Buffer = PBS
    • Sterile filtered
    • Does NOT contain sodium azide
    • Smallest Aliquot Size = 1 mg  (when in stock)
  • Conjugated MAbs: The facility will conjugate purified MAbs to fluorophores, HRP and biotin. 
  • Fragmented MAbs:  The facility will fragment most purified MAbs into FAbs or F(Ab')2 and purify them. 

 

All questions emailed to the facility will be answered within 1 business day. For more urgent issues, call:

  • Syeda Rizvi at 646-888-2331 (core facility member)
  • Anne Mui at 646-888-3157 (administrative assistant)

 

Please note: All antibodies are for RESEARCH PURPOSES ONLY

 

Mycoplasma Testing

To dermine if a cell culture contains mycoplasma, a sample of the media in which the cells are growing can be submitted to the facility for for testing by 10 am each Wednesday and the facility will test it using the MycoAlert-PlusTM kit (Lonza).  It employes a selective biochemical assay to detect the presence of conserved mycoplasmal enzymes (MycoAlert-PlusTM, Lonza) from live mycoplasma and covers a wide variety of Mycoplasma, Acholeplasma, and Ureaplasma species. 

It is imperitive that the samples be prepared in a manner that maximizes the number for live mycoplasma in the media to have confidence in a negative (no mycoplasma detected) result.  Please contact the faciity on how we recommend the samples be prepared.

All questions can be emailed to the facility and will be answered within 1 business day.  Alternative, you can call the facility staff at 646-888-2331.

 

Cell Line Distribution

ABCF has hundreds of cell lines (tumor, hybridomas, etc.) generated at Memorial Sloan Kettering Cancer Center (MSKCC) or The Rockefeller University (RU).  To search for lines in which you may be interested, go to the "Requsest Servcies" tab above, go to "Cell Line Distribution - Request Cell Line / Hybridoma" and once you have inititated an order there will be an excel file you can download.  Alternatively, email the facility at skiabcf@mskcc.org.

MSKCC and RU researchers can submit cell lines they developed, characterized and published with the ABCF and direct all requestors to the core to obtain the lines. This service includes:

    • Creation a comprehensive information sheet, which will be distributed with the cell line
    • Cell line authentication, which includes but may not be limited to:
      • STR Profiles (human lines only)
      • Mycoplama testing
      • Image of the cell line
      • Karyotyping
      • Flow cytometric analysis
    • Management of all Intellectual Property concerns (e.g. Material Transfer Agreements)
    • Ship the cells around the world

To order or submit cell lines, click on the "Requsest Servcies" tab above and choose either "Cell Line Distribution - Request Cell Line / Hybridoma" or "Cell Line Distribution - Submit a Cell Line / Hybridoma" respectively.
 

 

Core Facility Members and Contact Information

 __________________________________________

 

Frances Weis-Garcia, Ph.D.

Head, Bi-Institutional Monoclonal Antibody Core Facility

weisgarf@mskcc.org / 646-888-2354  

 

Anne Mui

Administrative Assistant

muia@mskcc.org / 646-888-3157

 

Syeda Rizvi

Senior Core Staff Member

Supports MAb and Mycoplasma Testing services

rizvis@mskcc.org / 646-888-2331

 

Lou Mattera

Core Staff Member

Supports custom MAb development

matteral@mskcc.org / 646-888-2331

 

Greg Davis

Core Staff Member

Supports custom MAb development

davisg@mskcc.org / 646-888-2331

 

Facility Email address:  skiabcf@mskcc.org

 

Locations

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Memorial Sloan Kettering Cancer Center

Mortimer B. Zuckerman Research Center  

Rooms 1553 and 1551

(646) 888-2331

 

The Rockefeller University

D.W. Bronk Laboratories Building

Room 415

(212) 327-7030

  

Links and Resources

 

MSKCC Tangible Research Material Database

Learn more about Memorial Sloan Kettering Cancer Center

Contacts

Name Role Phone Email Location
Frances Weis-Garcia
Head
 
(646) 888-2354
 
weisgarf@mskcc.org
 
MSKCC ZRC 1563
 
Anne Mui
Administrative Assistant
 
(646) 888-3157
 
muia@mskcc.org
 
MSKCC ZRC 1501
 
Syeda Rizvi
MAbs and Mycoplasma Testing
 
(646) 888-2331
 
rizvis@mskcc.org
 
MSKCC ZRC 1551/3
 
Lou Mattera
Custom MAb Development
 
(646) 888-2331
 
matteral@mskcc.org
 
MSKCC ZRC 1551/3
 
Greg Davis
Custom MAb Development
 
(646) 888-2331
 
davisg1@mskcc.org
 
MSKCC ZRC 1551/3
 



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Memorial Sloan Kettering Cancer Center / Zuckerman Research Center / 417 East 68th Street / New York City, New York 10065