Welcome!
The Antibody and Bioresource Core Facility is a
Bi-institutional shared resource supporting researchers at
Memorial Sloan Kettering Cancer Center
and The Rockefeller University
____________________________________________________________________________________________________________________
REMEMBER:
When publishing work done with or by the core, please
acknowledge that this shared resource is supported in part by the
NCI Cancer Center Support Grant (2 P30 CA008748-53)
and include the core's Research Resource Identifier (RRID: SCR_017691)
____________________________________________________________________________________________________________________
Services and Support
____________________________________________________________________________________________________________________
Custom Monoclonal Antibody Development
Researchers who cannot find an antibody that meets their scientific needs can partner off with the core to develop a strategy to generate custom monoclonal antibodies (MAbs). The facility is well equipped to:
- Provide expertise built on 20 years of experience
- Oversee new MAbs projects, as well as the validation of the MAb developed
- Immunize mice, rats and hamsters
- Create 10's of thousands of hybridomas by electrofusion
- Isolate the 1 - 15 % of IgG secreting hybridomas from the electrofusion product to minimize screening efforts and maintain them during screening
- Perform ELISAs
- Cryopreserve and store of hybridomas secreting antigen specific antibodies
- Subclone hybridoma cultures
The core does not develop polyclonal antibodies at this time but is available to provide advice free of charge.
With respect to general validation of antibodies, the core is here to lead researchers through the process and direct them to application experts in other core facilities as needed.
To speak with someone in the core about starting a custom MAb project, contact Frances Weis-Garcia by email or call 646-888-2354.
Monoclonal Antibodies
Monoclonal antibodies are available in various forms:
- Conditioned Media:
- Media from a hybridoma cultured at low density in a T flask
- 50 ml, 350 ml and 1,000 ml
- Typical MAb Concentration ~ 10 ug / ml
- Purified MAbs:
- MAbs purified from conditioned media
- Purity > 95%
- Buffer = PBS
- Sterile filtered
- Does NOT contain sodium azide
All questions emailed to the facility will be answered within one business day. For more urgent issues, call:
Please note: All antibodies are for RESEARCH PURPOSES ONLY.
- Brendan Buehler at 646-888-2331 for technical questions.
- Anne Mui at 646-888-3157 for administrative questions.
Mycoplasma Testing
To dermine if a cell culture contains mycoplasma, a sample of the media in which the cells are growing can be submitted to the facility for for testing by 10 am each Wednesday and the facility will test it using the MycoAlert-PlusTM kit (Lonza). It employes a selective biochemical assay to detect the presence of conserved mycoplasmal enzymes (MycoAlert-PlusTM, Lonza) from live mycoplasma and covers a wide variety of Mycoplasma, Acholeplasma, and Ureaplasma species.
It is imperitive that the samples be prepared in a manner that maximizes the number for live mycoplasma in the media to have confidence in a negative (no mycoplasma detected) result. Please contact the faciity on how we recommend the samples be prepared.
All questions can be emailed to the facility and will be answered within 1 business day. Alternative, you can call the facility staff at 646-888-2331.
Cell Line Distribution
ABCF has hundreds of cell lines (tumor, hybridomas, etc.) generated at Memorial Sloan Kettering Cancer Center (MSKCC) or The Rockefeller University (RU). To search for lines in which you may be interested, go to the "Requsest Servcies" tab above, go to "Cell Line Distribution - Request Cell Line / Hybridoma" and once you have inititated an order there will be an excel file you can download. Alternatively, email the facility at skiabcf@mskcc.org.
MSKCC and RU researchers can submit cell lines they developed, characterized and published with the ABCF and direct all requestors to the core to obtain the lines. This service includes:
To order or submit cell lines, click on the "Requsest Servcies" tab above and choose either "Cell Line Distribution - Request Cell Line / Hybridoma" or "Cell Line Distribution - Submit a Cell Line / Hybridoma" respectively.
Core Facility Members and Contact Information
__________________________________________
Frances Weis-Garcia, Ph.D.
Head, Bi-Institutional Monoclonal Antibody Core Facility
weisgarf@mskcc.org / 646-888-2354
Anne Mui
Administrative Assistant
muia@mskcc.org / 646-888-3157
Lou Mattera
Research Assistant
Supports custom MAb development
matteral@mskcc.org / 646-888-2331
Anthony Yasmann, Dr. rer. nat.
Senior Research Assistant
Supports custom MAb development
yasmanna@mskcc.org / 646-888-2331
Andrea Texcucano-Lazarte
Research Technician
Supports Cell Line and MAb services
texcuca@mskcc.org / 646-888-2331
Trevor Simon
Research Assistant
Supports custom MAb development
simont2@mskcc.org / 646-888-2331
Facility Email address: skiabcf@mskcc.org
Locations
__________________________________________
Memorial Sloan Kettering Cancer Center
Mortimer B. Zuckerman Research Center
Rooms 1553 and 1551
(646) 888-2331
The Rockefeller University
D.W. Bronk Laboratories Building
Room 415
(212) 327-7030
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Frances Weis-Garcia |
Head
|
(646) 888-2354
|
weisgarf@mskcc.org
|
MSKCC ZRC 1563
|
| Anne Mui |
Administrative Assistant
|
(646) 888-3157
|
muia@mskcc.org
|
MSKCC ZRC 1565
|
| Lou Mattera |
Custom MAb Development
|
(646) 888-2331
|
matteral@mskcc.org
|
MSKCC ZRC 1551/3
|
| Anthony Yasmann |
Custom MAb Development
|
(646) 888-2331
|
yasmanna@mskcc.org
|
MSKCC ZRC 1551/3
|
| Andrea Texcucano-Lazarte |
Cell Line Distritubion
|
(646) 888-2331
|
texcuca@mskcc.org
|
MSKCC ZRC 1551/3
|
| Trevor Simon |
Custom MAb Development
|
(646) 888-2331
|
simont2@mskcc.org
|
MSKCC ZRC 1551/3
|
Memorial Sloan Kettering Cancer Center / Zuckerman Research Center / 417 East 68th Street / New York City, New York 10065
